Isozim pada organ sporogoni Anopheles barbirostris van der wulp vektor dan non vektor

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Isozim pada organ sporogoni Anopheles barbirostris van der wulp vektor dan non vektor
Isozymes of the sporogony organs in Anopheles barbirostris van der wulp vector and non vector
JURNAL KEDOKTERAN YARSI 13 (3) : 256-262 (2005)
by
Ruben Dharmawan, Darukutni, Satimin Hadiwidjaja and Adi Prayitno
Sebelas Maret University School of Medicine, Surakarta.


ABSTRACT
The genetic control of malaria has not been completely successful as it needs more comprehensive understanding of the development of the parasite in the body of vector mosquitoes. This study aim is to reveal the possibility of isozymes in salivary glands and midguts of Anopheles barbirostris vector and non vector to be the factor influencing the growth and development of PIasmodium falciparum. The isozymes were detected in its natural and active forms using non dissociating electrophoresis. Populations selected were those from malaria endemic areas, i.e. one non vector population from Central Java and another one from Flores island. Bloodfed female mosquitoes were collected using human and animal bait collection. They were each colonized individually in the laboratory and their first filial (F1) mosquitoes were dissected to take their salivary glands and midguts out as materials for isozyme electrophoresis. Dissection and electrophoresis were carried out in low temperature to keep the isozyme activities. A total of eight isozymes were examined employing vertical polyacrylamide gel electrophoresis in respective buffer system, i.e. alcohol dehydrogenase (acd), glycerol dehydrogenase (gcd), phospoglucomutase (pgm), phosphoglucoisomerase (pgi), alpha esterase (a est), malic enzyme (me), leucine aminopeptidase (lap) and alkaline phosphatase (alp). Different electromorph patterns for vector and nonvector were observed in gcd, a est, me, lap and alp. It could be concluded that these isozymes might have some roles in the growth and development of Pl. falciparum in the salivary glands and midguts in addition to its classical use for the identification of sibling species. Further studies are still required before using this findings as alternative approach to control the growth and development of PI. falciparum in the body of vector mosquitoes.