Daya antibakteri madu terhadap beberapa kuman patogen secara in vitro

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Daya antibakteri madu terhadap beberapa kuman patogen secara in vitro
The antibacterial activity of honey against several pathogenic bacteria in vitro
JURNAL KEDOKTERAN YARSI 12 (3) : 041-046 (2004)
by
Lilis Suryani1 dan Nur Shani Meida2
1Department of Microbiology, Muhammadiyah University School of Medicine, Yogyakarta
2Department of Biochemistry, Muhammadiyah University School of Medicine Yogyakarta


ABSTRACT
Honey is a healthy liquid. Honey consists of many kinds of material that may recover human from infectious diseases, like antibiotics. Some infectious diseases like digestive diseases can be cured using honey. Microorganisms causing diseases are among others Staphylococcus aureus, Escherichia coli, Shigella dysenteriae, Vibrio cholerae and Salmonella typhi. This study is an experimental laboratory examination carried out to learn, the antibacterial activity of honey against Staphylococcus aureus, Escherichia coli, Shigella dysenteriae, Vibrio cholerae and Salmonella typhi. The antibacterial activity of honey has been tested by the determination of the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) applying the tube dilution method. The result of this study showed that the MIC and the MBC of honey against Staphylococcus aureus was about 16,6% and 33,33%, Salmonella typhi 18,88% and 37,76%, Escherichia coli 20,8% and 50%, Shigella dysenteriae 0,179% and 0,3585% and Vibrio cholerae 15,625% and 41,67% respectively. As a conclusion it was obvious that honey had indeed an antibacterial activity against Staphylococcus aureus, Salmonella typhi, Escherichia coli, Shigella dysenteriae and Vibrio cholerae res-pecttively. Honey had the highest antibacterial activity against Shigella dysenteriae while the lowest antibacterial activity was shown against Escherichia coli.