Fakultas Kedokteran YARSI
Aplikasi dendrimer sebagai sistem penghantar obat-obatan

PDF Cetak E-mail
Aplikasi dendrimer sebagai sistem penghantar obat-obatan
Dendrimer application as drug delivery system
JURNAL KEDOKTERAN YARSI 12 (2) : 096-106 (2004)
by
Yuhernita
Department of Chemistry, YARSI UNIVERSITY School of Medicdine Jakarta


ABSTRACT
Many studies in design, synthesis and polymer properties have been conducted to discover a new polymer that can be used as a drug delivery agent. Due to the improvement of drug targeting and therapeutic efficacy, various polymer carriers have been evaluated. However, the inability of drug or steric constrains of polymeric systems to reach the target site decreased drug activity. Dendritic polymers are considered alternative for the preparation of monodisperse drug carriers due to their well-defined structure, multivalency, and flexibility of functionalization. These unique properties have made their application in pharmaceutics and chemistry particularly attractive. This review elaborates the preparation of dendrimer and their potency for many applications, especially in drug delivery system.
 
The signal transduction of curcumin as anti inflammatory agent in cultured fibroblasts

PDF Cetak E-mail
The signal transduction of curcumin as anti inflammatory agent in cultured fibroblasts
JURNAL KEDOKTERAN YARSI 12 (3) : 001-006 (2004)
by
Sri Agus Sudjarwo
Department of Pharmacology, Airlangga University School of Medicine, Surabaya


ABSTRACT
In this study, the effects of curcumin on the activity of cyclooxygenase (COX) and Prostaglandin (PGE2) production were investigated in the fibroblast culture stimulated with 1 µM bradykinin. Curcumin significantly inhibited prostaglandin production in a dose-dependent manner in bradykinin-stimulated fibroblast. The inhibition of curcumin on PGE2 release was apparent at concentrations of 10 µg/ml, 20 µg/ml and 40 µg/ml. In the reverse transcriptase-polymerase chain reac-tion (RT-PCR) it was shown that COX-1 and COX-2 mRNA was expressed upon stimulation with bradykinin. The results suggest that mechanism of bradykinin in increasing PGE2 production in cultured fibroblast could be mediated by activation of COX-1 and COX-2 enzyme. We next examined the effect of curcumin on COX-1 and COX-2 enzymes using western blot analyses. As a result, curcumine inhibition on the activity of COX-1 was more potent than COX-2 enzyme upon stimulation with 1 µM bradykinin. The present study suggest that mechanism of curcumin as anti inflammatory agent is due to its ability to inhibit PGE2 production that could be mediated by inhibition to both COX-1 and COX 2 activity.
 
Komparasi respons tumor terhadap radiasi antara radioterapi dengan radioterapi plus vaksinasi BCG pada karsinoma nasofaring

PDF Cetak E-mail
Komparasi respons tumor terhadap radiasi antara radioterapi dengan radioterapi plus vaksinasi BCG pada karsinoma nasofaring
The comparison of tumor irradiation response between radiotherapy versus radiotherapy plus BCG vaccination in nasopharyngeal carcinoma
JURNAL KEDOKTERAN YARSI 12 (3) : 007-016 (2004)
by
Widodo Ario Kentjono
Departement of Otolaryngology, Airlangga University School of Medicine, Dr. Soetomo General Hospital, Surabaya


ABSTRACT
The presence of nasopharyngeal tumor, which is resistant to irradiation (radiotherapy), is unexpected, as it generally may develop to become recurrent tumor. Results of radiation therapy, presenting as lower irradiation tumor response, may possibly result from radiotherapy effect on immune system. Radiotherapy, which is primarily directed to eradicate nasopharyngeal carcinoma (NPC) cells, often results in the reduction of cellular immunity. Reduced quality of cancer immune surveillance, undertaken particularly by CD8 T cells (CTL), NK cells, activated macrophage and LAK cells, will be very disadvantageous since tumor growth may persist, and even becomes more progressive. BCG vaccine is known to increase immunological effector cells, whose activity is influenced by the cytokine of Th1 lymphocyte (Th1 response). BCG vaccination (as immunogenic stressor) given simultaneously with NPC radiotherapy is expected to increase cancer cells eradication in the nasopharynx. This study was aimed to prove that there was higher tumor irradiation response in nasopharyngeal carcinoma patients receiving radiotherapy plus BCG vaccination than that in patients receiving radiotherapy only. An experimental study (clinical trial) was carried out towards 77 patients, consisting of 38 patients in the control group and 39 patients in the treatment group. Radiotherapy was given in standard (conventional) dose and method, while BCG vaccination was administered by means of modified multiple scarification technique. Results revealed that significant difference was found between post-radiotherapy volume of nasopharyngeal tumor in the control group and that in the treatment group (p<0.05). Tumor irradiation response in those groups was also significantly different (p<0.05). In conclusion, BCG vaccination administered simultaneously with NPC radiotherapy might increase nasopharyngeal tumor response.
 
Pengaruh dekontaminasi dalam identifikasi Mycobacterium tuberculosis dengan pewarnaan Ziehl-Neelsen dan Polymerase Chain Reaction

PDF Cetak E-mail
Pengaruh dekontaminasi dalam identifikasi Mycobacterium tuberculosis dengan pewarnaan Ziehl-Neelsen dan Polymerase Chain Reaction
Effect of decontamination on the identification of Mycobac-terium tuberculosis by the Ziehl-Neelsen staining and Polymerase Chain Reaction
JURNAL KEDOKTERAN YARSI 12 (3) : 017-024 (2004)
by
Mochammad Hatta1,3, Eka Wisdawati2, Zaraswati Dwiyana2, Rosana Agus2, M. Sabir3, Yadi3, & Masyhudi4
1Department of Medical Microbiology, Hasanuddin University School of Medicine, Makassar
2Department of Microbiology, Hasanuddin University School of Sciences, Makassar
3Department of Medical Microbiology, Moslem University of Indonesia School of Medicine Makassar
4Department of Microbiology, Mulawarman University School of Medicine Samarinda


ABSTRACT
Ten sputum samples from suspects of pulmonary tuberculosis were collected and examined with and without decontamination technique, then staining by Ziehl-Neelsen and examined under the microscope. The results of undecontaminated samples were 7/10 (70%) positive of which 5/10 (50%) were positive 1, 2/10 (20%) was positive 3, and decontaminated samples were 10/10 (100%) positive consisting of 2/10 (20%) of positive 2, and 8/10 (80%) of positive 3. Three samples which were undecontaminated with negative results became positive after decontamination of which two samples were positive 2 and one sample was positive 3, and five samples which were positive 1 became positive 3 after decontamination. The number of acid-fast bacilli found before decontamination was less than that obtained after decontamination. The Polymerase Chain Reaction was carried out on the DNA of 3 samples representing Mycobacterium tuberculosis isolates applying the Boom method, which was amplificated further using the INS1-F and the INS2-R primers and later on followed, by electrophoresis. The results of the undecontaminated samples were 2/3 (66.7%) positives, and the decontaminated samples were 3/3 (100%) positives. In conclusion, the results of staining applying the Ziehl-Neelsen technique and the Polymerase Chain Reaction was better observed in those after decontamination than those without decontamination.
 
Penampilan Fragile site dalam dua media kultur yang berbeda

PDF Cetak E-mail
Penampilan Fragile site dalam dua media kultur yang berbeda
Fragile site expression in two different culture media
JURNAL KEDOKTERAN YARSI 12 (3) : 025-032 (2004)
by
Dwi Ngestiningsih1, Antonius Eko Prasetyo2, Sultana MH. Faradz3, Achmad Zulfa Juniarto4
1Department of Biochemistry, DIPONEGORO UNIVERSITY School of Medicine, Semarang
2Student of DIPONEGORO UNIVERSITY School of Medicine, Semarang
3Department of Histology/Cytogenetic and Molecular Genetic Unit, Medical Biotechnology Laboratory, DIPONEGORO UNIVERSITY School of Medicine, Semarang
4Department of Biology, DIPONEGORO UNIVERSITY School of Medicine, Semarang


ABSTRACT
The fragile X syndrome is the most common cause of inherited mental retardation. Cytogenetic analysis showed that fragile X syndrome was associated with fragile site at the end of long arm chromosome X (Xq27.3). To express fragile site on X chromosome the cell culture media have to be deficient in folic acid or otherwise use folate inhibitor. The aim of this study was to screen and distinguish fragile site expression on mentally retarded person that cultured with folate depleted media and use of folate inhibitor. This study was a descriptive observational method. Twenty-seven samples with unexplained mental retardation were selected from Dharma Putra Special School Semin by limited physical examination to exclude Down syndrome, multiple malformation and deaf-mute. Blood from every sample was cultured in two different media namely TC 199 and MEM with thymidine. The result of all samples that were cultured in TC 199 and MEM with thymidine showed fragile site on Xq27.3 for 11 samples with average frequency 16.82% and 1 sample with average frequency 5% respectively. The other major finding of this study was that fragile X syndrome was the main cause of mental retardation in this region.
 
Struktur histologik ginjal tikus (Rattus norvegicus L.) galur Sprague Dawley setelah pencekokan spent catalyst lokal dan impor dari Residual Catalytic Cracking Unit

PDF Cetak E-mail
Struktur histologik ginjal tikus (Rattus norvegicus L.) galur Sprague Dawley setelah pencekokan spent catalyst lokal dan impor dari Residual Catalytic Cracking Unit
Histological structure of rat kidney (Rattus norvegicus L.) Sprague Dawley strain after being induced by local and import spent catalyst from Residual Catalytic Cracking Unit
JURNAL KEDOKTERAN YARSI 12 (3) : 033-040 (2004)
by
Etty Widayanti
Department of Biology, YARSI UNIVERSITY School of Medicine, Jakarta


ABSTRACT
The previous experiments had reported that spent catalyst from Residual Catalytic Cracking UP VI Balongan, could damage histological structure of rat kidney in the dosage of more than 3,75 g/kg bw for 21 days (sub acute). The purpose of this study was to investigate the effect of the local and imported suspensions spent catalyst on the histological structure of rat kidney in the dosage 0; 0,1; 0,3; 1; and 3 g/kg bw for 56 days (sub chronic). The result of Anava test (? = 0,05) for glomerulus diameter, urinary space distance, and Bowman’s capsule diameter in all dosages showed no statistical difference, nor did histological examination result.
 
Daya antibakteri madu terhadap beberapa kuman patogen secara in vitro

PDF Cetak E-mail
Daya antibakteri madu terhadap beberapa kuman patogen secara in vitro
The antibacterial activity of honey against several pathogenic bacteria in vitro
JURNAL KEDOKTERAN YARSI 12 (3) : 041-046 (2004)
by
Lilis Suryani1 dan Nur Shani Meida2
1Department of Microbiology, Muhammadiyah University School of Medicine, Yogyakarta
2Department of Biochemistry, Muhammadiyah University School of Medicine Yogyakarta


ABSTRACT
Honey is a healthy liquid. Honey consists of many kinds of material that may recover human from infectious diseases, like antibiotics. Some infectious diseases like digestive diseases can be cured using honey. Microorganisms causing diseases are among others Staphylococcus aureus, Escherichia coli, Shigella dysenteriae, Vibrio cholerae and Salmonella typhi. This study is an experimental laboratory examination carried out to learn, the antibacterial activity of honey against Staphylococcus aureus, Escherichia coli, Shigella dysenteriae, Vibrio cholerae and Salmonella typhi. The antibacterial activity of honey has been tested by the determination of the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) applying the tube dilution method. The result of this study showed that the MIC and the MBC of honey against Staphylococcus aureus was about 16,6% and 33,33%, Salmonella typhi 18,88% and 37,76%, Escherichia coli 20,8% and 50%, Shigella dysenteriae 0,179% and 0,3585% and Vibrio cholerae 15,625% and 41,67% respectively. As a conclusion it was obvious that honey had indeed an antibacterial activity against Staphylococcus aureus, Salmonella typhi, Escherichia coli, Shigella dysenteriae and Vibrio cholerae res-pecttively. Honey had the highest antibacterial activity against Shigella dysenteriae while the lowest antibacterial activity was shown against Escherichia coli.
 
Proliferasi splenosit mencit setelah imunisasi intranasal protein terlarut Toxoplasma gondii

PDF Cetak E-mail
Proliferasi splenosit mencit setelah imunisasi intranasal protein terlarut Toxoplasma gondii
Proliferation of mice splenocytes after intranasal immunization with soluble protein of Toxoplasma gondii
JURNAL KEDOKTERAN YARSI 12 (3) : 047-053 (2004)
by
Lilik Wijayanti1, Marsetyawan HNES2, Supargiyono2, Widya Asmara3, Wayan T Artama3, Susi Iravati4, Adi Prayitno1
1Sebelas Maret University School of Medicine, Surakarta
2Gadjah Mada University School of Medicine, Yogyakarta
3Gadjah Mada University School of Veterinary, Yogyakarta
4Gadjah Mada University School of Pharmacy, Yogyakarta


ABSTRACT
Infection of Toxoplasma gondii (T. gondii) may cause problems in pregnancy (e.g. abortion, stillbirth or congenital toxoplasmosis). Both humoral and cellular immune responses can be observed during T. gondii infection. This infection induces strong cellular immune responses leading to proliferation of lymphocytes. This study was aimed at studying the effect of immunization via intranasal with soluble protein on cellular immune response. Three groups of Balb/c mice were used throughout the study. The first group was immunized with 10 µg soluble protein, the second group with 10 µg soluble protein plus 0,5 µg labile toxin (LT) and the third group was used as control. Proliferative activity of splenocyte was examined before and after immunization with 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The result showed that the proliferative index of splenocyte in immunized mice was increase after immunization. Statistical analysis showed a significant difference (p = 0,01) between each group. In the second group the proliferation index of splenocyte was greater than that of the other group.
 
Isolasi Salmonella typhi dari penderita demam tifoid

PDF Cetak E-mail
Isolasi Salmonella typhi dari penderita demam tifoid
Isolation of Salmonella typhi from typhoid fever patients
JURNAL KEDOKTERAN YARSI 12 (3) : 054-060 (2004)
by
Hera Noviana
Department of Microbiology, Atma Jaya Catholic University School of Medicine, Jakarta


ABSTRACT
Salmonella typhi is the etiologic agent of typhoid fever. This bacterium is only pathogenic for humans, which can be cultured from blood and stools. Most of the infections have the same clinical features, so it is difficult to diagnose exactly typhoid fever. Antimicrobial drugs are the first choice therapy for typhoid fever patients so that antibiotics susceptibility tests is very important to be carried out to control the resistance level of the bacterial isolates. During a period from July, 2000 to September, 2003, a number of ten milliliters of blood was collected from patients with typhoid fever to be examined using bile-broth (217 samples), conventional blood culture with thioglycollate medium (75 samples), and applying the BACTEC system (224 samples), all carried out at the Clinical Microbiology Laboratory, Medical Faculty, Atmajaya University. From a total of 516 samples 16 strains of Salmonella typhi (3,10%), 5 strains of Salmonella paratyphi A (0,97%), and 36 other species of bacteria were isolated. Antimicrobial drugs with the best antimicrobial activities were meropenem, cefotaxime, ceftriaxone, cefixime, and amoxillin+clavulanic acid
 
<< Awal < Sebelumnya 11 12 13 14 15 16 17 18 19 Berikutnya > Akhir >>

JPAGE_CURRENT_OF_TOTAL