The signal transduction of curcumin as anti inflammatory agent in cultured fibroblasts

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The signal transduction of curcumin as anti inflammatory agent in cultured fibroblasts
JURNAL KEDOKTERAN YARSI 12 (3) : 001-006 (2004)
by
Sri Agus Sudjarwo
Department of Pharmacology, Airlangga University School of Medicine, Surabaya


ABSTRACT
In this study, the effects of curcumin on the activity of cyclooxygenase (COX) and Prostaglandin (PGE2) production were investigated in the fibroblast culture stimulated with 1 µM bradykinin. Curcumin significantly inhibited prostaglandin production in a dose-dependent manner in bradykinin-stimulated fibroblast. The inhibition of curcumin on PGE2 release was apparent at concentrations of 10 µg/ml, 20 µg/ml and 40 µg/ml. In the reverse transcriptase-polymerase chain reac-tion (RT-PCR) it was shown that COX-1 and COX-2 mRNA was expressed upon stimulation with bradykinin. The results suggest that mechanism of bradykinin in increasing PGE2 production in cultured fibroblast could be mediated by activation of COX-1 and COX-2 enzyme. We next examined the effect of curcumin on COX-1 and COX-2 enzymes using western blot analyses. As a result, curcumine inhibition on the activity of COX-1 was more potent than COX-2 enzyme upon stimulation with 1 µM bradykinin. The present study suggest that mechanism of curcumin as anti inflammatory agent is due to its ability to inhibit PGE2 production that could be mediated by inhibition to both COX-1 and COX 2 activity.